منابع مشابه
Live imaging of mouse embryos.
The development of the mouse embryo is a dynamic process that requires the spatial and temporal coordination of multiple cell types as they migrate, proliferate, undergo apoptosis, and differentiate to form complex structures. However, the confined nature of embryos as they develop in utero limits our ability to observe these morphogenetic events in vivo. Previous work has used fixed samples an...
متن کاملTopic Introduction Live Imaging of Mouse Embryos
The development of the mouse embryo is a dynamic process that requires the spatial and temporal coordination of multiple cell types as they migrate, proliferate, undergo apoptosis, and differentiate to form complex structures. However, the confined nature of embryos as they develop in utero limits our ability to observe these morphogenetic events in vivo. Previous work has used fixed samples an...
متن کاملIn ovo live imaging of avian embryos.
Vertebrate development is best studied in an intact embryo model, but a robust interface between time-lapse microscopy and in vivo embryo health and maintenance can be difficult to achieve in model systems that rely on external factors for life support. This protocol presents a system for in ovo culture and time-lapse imaging of fluorescently labeled cells within living avian embryos, using a T...
متن کاملLive Imaging of Whole Mouse Embryos during Gastrulation: Migration Analyses of Epiblast and Mesodermal Cells
During gastrulation in the mouse embryo, dynamic cell movements including epiblast invagination and mesodermal layer expansion lead to the establishment of the three-layered body plan. The precise details of these movements, however, are sometimes elusive, because of the limitations in live imaging. To overcome this problem, we developed techniques to enable observation of living mouse embryos ...
متن کاملLabel-free subcellular 3D live imaging of preimplantation mouse embryos with full-field optical coherence tomography.
Early patterning and polarity is of fundamental interest in preimplantation embryonic development. Label-free subcellular 3D live imaging is very helpful to its related studies. We have developed a novel system of full-field optical coherence tomography (FF-OCT) for noninvasive 3D subcellular live imaging of preimplantation mouse embryos with no need of dye labeling. 3D digitized embryos can be...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2011
ISSN: 1559-6095
DOI: 10.1101/pdb.top104